Figure 3.
PCH-2::GFP-3XFLAG localizes to unattached kinetochores during spindle checkpoint activation. (A) An immunoblot of PCH-2::GFP-3XFLAG worms shows that the full-length tagged protein is expressed. (B, left) PCH-2:::GFP-3XFLAG is expressed in the mitotic embryo. The arrowhead (left) indicates enrichment of PCH-2::GFP-3XFLAG after NEBD in the AB cell, whereas the single arrow (right) indicates PCH-2::GFP-3XFLAG exclusion from the nucleoplasm before mitotic entry in the P1 cell. Bar, 10 µm. (B, right) Checkpoint activation via RNAi of zyg-1 localizes PCH-2::GFP-3XFLAG to unattached kinetochores. Bar, 5 µm.
