Effects of inhibitors on fin-like protrusion properties. (A) Kymographs from cells treated with different inhibitors showing the effects on the formation and propagation of fins. (B and D) Quantification of the (B) linear speed (error bar, SEM from a typical experiment; NB, 33 [control], 32 [blebbistatin (Bleb)], 30 [latrunculin A.], 38 [CK666], 33 [NSC23766], 37 [Y27632]); (C) frequency of the protrusions (NB, 14 [control], 10 [blebbistatin], 10 [latrunculin A.], 10 [CK666], 12 [NSC23766], 12 [Y27632]); and (D) velocity of the protrusions (NB, 59 [control], 49 [blebbistatin], 27 [CK666], 59 [NSC23766], 54 [Y27632]) in different conditions tested. (E and F) Cells were incubated with blebbistatin or Y27632 inhibitor. Oversized and normal fins were formed (arrowheads). Bar, 10 µm.