The dynein MTBD is dispensable for plus end targeting but is required for Num1_CC-mediated plus end depletion. (A) Schematic representation of the Dyn1_ΔMTBD mutant. (B) Representative images of GAL1p:num1_CC cells expressing mTurquoise2-Tub1 and Dyn1_ΔMTBD-3mCherry used for quantitation in C and D. (C) The percentage of cells that exhibit plus end (red) or SPB (green) Dyn1_ΔMTBD-3mCherry foci is plotted for cells shown in B. Error bars represent the standard error of proportion (n ≥ 126 cells). (D) Box plot of fluorescence intensity values of plus end–associated Dyn1_ΔMTBD-3mCherry (n ≥ 40 foci). (E and F) Representative images of GAL1p:num1_CC dyn1_ΔMTBD cells expressing mTurquoise2-Tub1 and either Jnm1- (E) or Nip100-3mCherry (F) used for quantitation in G and H. (G) The percentage of dyn1_ΔMTBD cells that exhibit plus end or SPB Jnm1- or Nip100-3mCherry foci is plotted for GAL1p:num1_CC cells grown in glucose (−Num1_CC) or galactose (+Num1_CC; n ≥ 100 cells). (H) Box plot of fluorescence intensity values of plus end–associated Jnm1- or Nip100-3mCherry (n ≥ 56 foci). For all box plots, whiskers define the range of data, boxes encompass the 25th to 75th quartiles, the line depicts the median value, and the “x” depicts the mean value. All images are maximum-intensity projections of a 2-µm Z-stack of wide-field images. Arrows indicate plus end foci. Bars, 2 µm. See also Fig. S5.