Interaction between dynein and Num1_CC is required for plus end depletion. (A) Schematic representation of the Num1_CC^LL/EE mutant. (B) Representative images of GAL1p:num1_CC^LL/EE and GAL1p:num1_CC cells expressing mTurquoise2-Tub1 and Dyn1-3mCherry used for quantitation in C. (C) The percentage of cells that exhibit plus end (red) or SPB (green) fluorescent foci is plotted for the strains overexpressing wild-type (WT) or mutant (LL/EE) Num1_CC. Error bars represent the standard error of proportion (n ≥ 170 cells). (D) Diagram depicting dynein N-terminal (tail) and C-terminal (motor) domains and an artificially dimerized GST-Dyn1_MOTOR construct (see text). (E) Representative images of GAL1p:num1_CC cells expressing mTurquoise2-Tub1 and Dyn1_MOTOR-3YFP used for quantitation in F. (F) The percentage of cells that exhibit plus end (red) or SPB (green) fluorescent Dyn1_MOTOR-3YFP or GST-Dyn1_MOTOR-3mCherry foci is plotted. Error bars represent the standard error of proportion (n ≥ 87 cells). All images are maximum-intensity projections of a 2-µm Z-stack of wide-field images. Arrows indicate plus end foci, and the arrowhead indicates the SPB focus. Bars, 2 µm.