Membranous ARF6 and MT1-MMP and KIF5B and KIF3A expression in breast cancer. (A–D) Expression of membranous MT1-MMP (A), membranous ARF6 (B), KIF5B (C), and KIF3A (D) was measured by IHC on TMA (example of score assignment). (E) Expression of MT1-MMP, ARF6, KIF5B, and KIF3A segregated in low- and high-expression classes is significantly increased in tumor component of IDCs as compared with adjacent peritumoral area. Values are shown as percentage of total tumors. *, membranous signal was considered. (F and G) Box plot representation with maximum and minimum, 75th and 25th percentiles, and median values of H score of the different markers correlating with the molecular subtypes (F) and pathological grades (G). Analysis was performed on 311 cases for which scores were available for all four markers. ARF6 and MT1-MMP plasma membrane H scores and total (cytosol + plasma membrane) KIF5B and KIF3A H scores were considered.