Hypoxia and reduction of intracellular ATP levels acutely deplete inositol phospholipids on PM. (A) Hypoxia reversibly inhibits PM targeting of PLCδ-PH::GFP, GRP1-PH::GFP, and Numb::GFP in Drosophila follicular cells (n = 4). (B) Inhibition of ATP production by 2-DG and AM in HEK293 cells induced loss of PLCδ::PH::GFP from PM. ***, P < 0.001. Bars, 5 µm. Error bars represent means ± SEM.