Lgl membrane targeting does not require intact actin cytoskeleton. (A) Drosophila embryos of lgl::RFP MoeAD::GFP were treated with 10 µM latrunculin-A (LAT) with 0.5% ethanol (EtOH) as control. Each pair of control and treated samples was captured under identical imaging parameters and processed identically. Actin was visualized by MoeAD::GFP (n = 4). (B) MDCK cells were treated with 25 µg/ml cytochalasin D (CytoD), with 0.5% ethanol as control. Actin was visualized by TRITC-phalloidin staining (n = 4). Each pair of control and treated samples was captured under identical imaging parameters and processed identically. **, P < 0.01; ***, P < 0.001. ns, not significant. Bars, 5 µm. Error bars represent means ± SEM.