Hypoxia and reduction of intracellular ATP levels inhibit the PM targeting of Lgl in both Drosophila and mammalian cells. (A) lgl::GFP embryos were treated with PBS (control) or 0.5% cyanide (CN). Embryos were double labeled by anti-Baz (red) and anti-GFP or anti-HA (green) antibodies (n = 6). (B) Dissected ovaries from lgl::GFP females that were treated with 1 µM AM or 0.5% cyanide (n = 6). PBS and 1% DMSO served as controls. (C) Inhibition of mLgl::GFP PM targeting in hypoxia-treated MDCK or HEK293 cells (n = 4). (D) Inhibition of ATP production by 2-DG and AM in HEK293 cells induced loss of mLgl::RFP from PM that can be reversed when drugs were washed out. PM localization of GAP::GFP is unaffected (n = 4). *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns, not significant. Bars, 5 µm. Error bars represent means ± SEM.