Bld10 is required for GFP-Root localization to brain and testis centrosomes/centrioles but is dispensable for rootlet assembly in ciliated neurons. (A) bld10 null mutant abolishes GFP-Root localization to centrosomes in NBs and ganglion mother cells (GMCs). The mother and the daughter centrosomes in the NB are distinguished by the pericentriolar material protein Cnn, which distributes more in the daughter than the mother. (B) bld10 mutant abolishes GFP-Root localization to centrioles in mature spermatocytes and spermatids, though some polar spermatocytes still have GFP-Root localizing at the centrioles (arrows). γ-Tub marks the centrioles. (C) In the bld10 JO or leg EsOs, GFP-Root localization to rootlets appears unaffected, with normal length and morphology (arrows). Actin marks scolopale rods. (D) bld10 (null) mutant flies show normal climbing activities in the negative geotaxis assay. Numbers of males assayed are indicated inside the bars. ns, P > 0.05. Bars: (A and B) 10 µm; (zoom) 500 nm; (C) 10 µm. See also Fig. S5.