P2Y2 receptors trigger mTORC2 signaling. Differentiated HL-60 cells were pretreated with or without 100 µM suramin (A) for 5 min and stimulated with (B) 100 nM fMLP or 10 µM UTP for the indicated periods of time, and activation of MAPK p38, mTORC1, and mTORC2 signaling was determined by immunoblotting as described in Fig. 5. Total MAPK p38 antibodies were used as a protein loading control. Error bars represent means ± SD (error bars) of at least three independent experiments; *, P < 0.05, compared with corresponding control; Student’s t test.