FPR and P2Y2 trigger mTOR phosphorylation and mitochondrial activation. (A) Differentiated HL-60 cells were stimulated for 30 s with 1 µM fMLP, 100 µM ATP, or 100 µM UTP, and mTOR and MAPK p38 activation was determined by immunoblotting with phosphospecific anti-mTOR and anti–MAPK p38 antibodies. Antibodies recognizing total MAPK p38 were used to verify equal protein loading. (B) Freshly isolated PMNs were loaded with 1 µM Rhod2 for 5 min, pretreated or not (control) with 100 µM suramin, and stimulated with fMLP, ATP, or UTP for 1 min, and mitochondrial Ca²⁺ uptake was estimated with flow cytometry. Results are expressed as means ± SD (error bars) and are representative of at least three independent experiments; one-way ANOVA; *, P < 0.05.