Figure 3.
Figure 3. Activated mitochondria with higher Δψm accumulate at the front of cells. (A) PMNs were plated onto fibronectin-coated glass coverslips and stained with 100 ng/ml JC-1 for 15 min, and the Δψm was recorded in real time using fluorescence microscopy (DMI6000 B; Leica; objective: 100× oil, NA 1.30; Spot Boost EMCCD camera, EM 150; bar, 5 µm; see also Video 4). (B) The change in JC-1 red fluorescence was analyzed separately for mitochondria at the front and back of cells. (C) The change in the percentage of mitochondria with high versus low membrane potential at the front of cells is shown at different times after fMLP stimulation. Data represent means ± SD (error bars) of normalized gray values of 15–25 cells. Statistical analysis was done with one-way ANOVA; *, P < 0.05.

Activated mitochondria with higher Δψm accumulate at the front of cells. (A) PMNs were plated onto fibronectin-coated glass coverslips and stained with 100 ng/ml JC-1 for 15 min, and the Δψm was recorded in real time using fluorescence microscopy (DMI6000 B; Leica; objective: 100× oil, NA 1.30; Spot Boost EMCCD camera, EM 150; bar, 5 µm; see also Video 4). (B) The change in JC-1 red fluorescence was analyzed separately for mitochondria at the front and back of cells. (C) The change in the percentage of mitochondria with high versus low membrane potential at the front of cells is shown at different times after fMLP stimulation. Data represent means ± SD (error bars) of normalized gray values of 15–25 cells. Statistical analysis was done with one-way ANOVA; *, P < 0.05.

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