Megator and soluble tubulin accumulate in the spindle region independently of MTs. (A) S2 cell expressing Megator-mCherry and GFP–α-tubulin upon MT depolymerization with colchicine before NEB. (B) Normalized relative fluorescence (nuclear/cytoplasm) of GFP–α-tubulin and Megator-mCherry. The relative fluorescence was set to 1 at time 0 (maximal accumulation of soluble tubulin in the nuclear region). Data points represent means obtained from seven cells, to which an exponential curve was fit. Error bars represent standard deviations. (C) Accumulation of GFP–α-tubulin and Megator-mCherry in the nuclear region after NEB in an unperturbed mitosis. Time is given in hours:minutes:seconds, relative to NEB. Bars, 10 µm.