E-cadherin down-regulation partially rescues purse string formation when endocytosis is blocked. (A and B) Epidermal cells in embryos injected with water (A) or shg dsRNA (B) and immunostained for E-cadherin. (C) Relative junctional E-cadherin fluorescence with respect to the counterstain (Dlg) in yellow white embryos injected with water (blue; n = 7) or 27.5 ng/µl shg dsRNA (red; n = 10). (D and E) Epidermal cells in shi^ts1; GFP:moesin embryos at the restrictive temperature (35°C), injected with water (D) or shg dsRNA (E). Time after wounding is indicated. Red lines represent wound sites. Yellow arrowheads point at actin-rich regions at the wound margin. (A, B, D, and E) Anterior left, dorsal up. Bars, 5 µm. (F) Mean wound area in shi^ts1 embryos at the restrictive temperature, injected with water (blue; n = 9) or shg dsRNA (red; n = 11). (G) Quantification of actin-rich structures at the wound margin, represented as the 90th percentile of the actin fluorescence around the wound. (H) Quantification of protrusive activity at the wound margin. Error bars, SEM; *, P < 0.05; ***, P < 0.001.