Figure 4.
Figure 4. Recruitment of cortactin to late endosomes depends on Arp2/3 complex activity. (A) Representative images show cortactin (green), actin (red), and Rab7 (blue) localization after 2 h treatment of MDA-MB-231 cells with 800 nM YM201636 ± 200 µM CK-666. Bars, 20 µm (5 µm for magnifications). (B) Images from cells treated with YM201636 and 100 or 200 µM CK-666 were analyzed for percentage of colocalization of cortactin or actin with Rab7. The DMSO and YM201636 datasets (no CK-666) are the same data as that shown in Fig. 3 ( C and D). Data shown as box and whiskers plots with the box indicating the 25th and 75th percentiles, solid line indicating the median, and whiskers indicating the 95% confidence intervals. 3 independent experiments, n ≥ 50 cells for each condition. ****, P < 0.0001.

Recruitment of cortactin to late endosomes depends on Arp2/3 complex activity. (A) Representative images show cortactin (green), actin (red), and Rab7 (blue) localization after 2 h treatment of MDA-MB-231 cells with 800 nM YM201636 ± 200 µM CK-666. Bars, 20 µm (5 µm for magnifications). (B) Images from cells treated with YM201636 and 100 or 200 µM CK-666 were analyzed for percentage of colocalization of cortactin or actin with Rab7. The DMSO and YM201636 datasets (no CK-666) are the same data as that shown in Fig. 3 ( C and D). Data shown as box and whiskers plots with the box indicating the 25th and 75th percentiles, solid line indicating the median, and whiskers indicating the 95% confidence intervals. 3 independent experiments, n ≥ 50 cells for each condition. ****, P < 0.0001.

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