AJs interacting with actin through vinculin produce static junctions. (a and b) Immunofluorescence microscopy of 468 cells expressing the α-catenin deletion mutant, Dn-αCat(1–505). Cells were double stained for Dendra2 (Dn) and actin (Act; a) or Dn and vinculin (Vin; b). (a) Apicolateral AJs are associated with fine actin structures (apical focus plane). The basolateral junctions were also present but not associated with actin bundles (arrows, basal focus plane). (b) Apicolateral and lateral junctions both recruit vinculin. Bars, 10 µm. (c) Dendra photoconversion assay of the apicolateral AJs in 468 cells expressing Dn-αCat, Dn-αCat-ΔVin, or Dn-αCat(1–505). The intensity of the red fluorescence in the photoconverted spots decreases over time. The error bars represent SEs (n = 20).