Phagosome ingestion and transport. (A) Time-lapse of 3D-reconstructed images, showing the ingestion of POSs (red) at the apical surface of an RPE cell expressing GFP (green). Each subsequent image represents a time lapse of 62 s (Video 1). (B) Time-lapse images of a primary mouse RPE cell in which microtubules are labeled with GFP–α-tubulin, and ingested POSs are labeled with Texas red. The track of one POS phagosome is shown in the enlarged image (right); different locations of the phagosome are indicated with white or blue spots, depending on the direction of the movement. Each subsequent image represents a time lapse of 2 s (Video 2). Bars: (A) 5 µm; (B) 2 µm.