MTM-1 regulates PtdIns3P accumulation on phagosomes. (A) Time-lapse images of a cell corpse in a wild-type embryo expressing both mCHERRY::MTM-1(C378S) and GFP::2xFYVE. “0 min” represents the time point when MTM-1(C378S) was first detected around the cell corpse. The time period until the cell corpse was surrounded by MTM-1(C378S) was defined as the pseudopod extension stage. The pseudopod extension time and duration of MTM-1(C378S) and 2xFYVE were quantified. (B and C) Time-lapse images of cell corpses in wild-type embryos coexpressing mCHERRY::MTM-1(C378S), GFP::2xFYVE, and PPK-1 (B) or mCHERRY::MTM-1 and YFP::2xFYVE (C). (D and E) MTM-1(C378S) duration and 2xFYVE labeling with or without PPK-1 expression are quantified in D. MTM-1(C378S) and MTM-1 duration and 2xFYVE labeling are quantified in E. (F and G) Time-lapse images of germ cell corpses in gla-3RNAi (F) or ocrl-1RNAi (G) animals coexpressing mCHERRY::MTM-1(C378S) and YFP::2xFYVE. The pseudopod extension time and duration of MTM-1(C378S) and 2xFYVE are shown beneath the images. In ocrl-1RNAi animals, most phagosomes (75%, arrowheads) were not labeled by 2xFYVE during the time-lapse analysis, whereas all phagosomes followed in gla-3RNAi were positive for 2xFYVE. (A and D–G) “n” indicates the number of quantified phagosomes. (A–C, F, and G) Images in 20–25 z series were captured. Representative images are shown. Data are shown as mean ± SD. Bars, 5 µm.