Apoptotic cell–containing phagosomes are arrested at the PtdIns(4,5)P2-positive stage in mtm-1 and ocrl-1 RNAi worms. DIC and fluorescent images of the gonads in gla-3RNAi, mtm-1RNAi, or ocrl-1RNAi animals expressing GFP::PLCδ1-PH (A–B’, Q, and Q’), GFP::RAB-5 (E–F’, S, and S’), GFP::RAB-7 (G–H’, T, and T’), NUC-1::nCHERRY (I–J’, U, and U’), LST-4::GFP (K–L’, V, and V’), and DYN-1::GFP (M–N’, W, and W’) or stained by LysoSensor green (C–D’, R, and R’). White arrowheads and boxes indicate germ cell corpses labeled by phagosomal markers, and yellow ones designate unlabeled corpses. Quantification is shown in O and X. (P) Germ cell corpses were quantified in controlRNAi and ocrl-1RNAi worms at 48 h after L4/adult molt. (O, P, and X) At least 15 animals were scored in each strain, and data are shown as mean ± SD. Recruitment of each phagosomal marker in mtm-1(RNAi) or ocrl-1(RNAi) was individually compared with that in the control group by unpaired t tests. **, P < 0.0001. Bars, 10 µm.
