Figure 4. Ecad cis-oligomerization... | Rockefeller University Press

Figure 4.

$Figure 4. Ecad cis-oligomerization stabilizes cadherin–catenin complexes at cell–cell contacts. (A) Characteristic images of GFP and mCherry signal before (Pre-bleach), immediately after bleaching (Bleach), and 250 s after the bleach (Post-bleach) performed on wt Ecad-GFP and α-catenin-mCherry doubly transfected cells. White squares represent the bleached region. Bar, 20 µm. (B) Normalized fluorescence recovery curves versus time for wt Ecad-GFP, cis-Ecad-GFP, and α-catenin-mCherry in wt Ecad and cis-Ecad expressing cells (n ≥ 29). (C) Box and whiskers plots (median + 10–90%) showing the mobile fraction (left) and the t1/2 (right) extracted from a one-exponential decay fit of fluorescence recovery curves. The disruption of the cis-interface led to a moderate increase of the mobile fraction of Ecad and α-catenin molecules without apparent modification of the diffusion characteristic times. *, P < 0.02; **, P < 0.05; ns, not significant (paired Student’s t test; n ≥ 23).$

Ecad cis-oligomerization stabilizes cadherin–catenin complexes at cell–cell contacts. (A) Characteristic images of GFP and mCherry signal before (Pre-bleach), immediately after bleaching (Bleach), and 250 s after the bleach (Post-bleach) performed on wt Ecad-GFP and α-catenin-mCherry doubly transfected cells. White squares represent the bleached region. Bar, 20 µm. (B) Normalized fluorescence recovery curves versus time for wt Ecad-GFP, cis-Ecad-GFP, and α-catenin-mCherry in wt Ecad and cis-Ecad expressing cells (n ≥ 29). (C) Box and whiskers plots (median + 10–90%) showing the mobile fraction (left) and the t_1/2 (right) extracted from a one-exponential decay fit of fluorescence recovery curves. The disruption of the cis-interface led to a moderate increase of the mobile fraction of Ecad and α-catenin molecules without apparent modification of the diffusion characteristic times. *, P < 0.02; **, P < 0.05; ns, not significant (paired Student’s t test; n ≥ 23).