Figure 9.
Localization of PLP to satellites requires Cnn CM2. Embryos were stained for the indicated proteins and imaged by confocal microscopy (A) or SIM (B). PLP satellites (orange arrows) are present in all genotypes but cnnB4 mutants, which resemble mitotic centrosomes. The PLP centriole pool is present in all genotypes (blue arrows). plp− and cnnB4 mutants do not properly assemble PCM around the centriole (brackets). Arrowheads show a cytoplasmic particle or rare PLP satellite in cnnB4 mutant. Bars: (A) 2.5 µm; (B) 1 µm.