PLP is required for MT organization. (A and B) WT and plp⁻ mitotic (A) and interphase (B) embryos stained for the indicated proteins. (A’) Acentriolar Cnn particle (inset) organizes MTs. Boxes in B show radial MT array, enlarged in the insets on the right. (C and D) Live GFP-MT in embryos. Broken circles show MTOC inactivation. Arrows show nuclear collisions and the arrowhead shows an orthogonal spindle. (E) Centrosome separation (closed arrowhead) and detachment (open arrowhead) defects are quantified in F. (G) Centrosome and nucleus positioning defects with two nuclei (asterisk) and more than two centrosomes (numerals) per pseudo-cell are quantified in H and F, and show mean ± SD (error bars). ***, P < 0.0001. Data shown are from a single representative experiment out of two repeats. Bars: (A and B, main panels) 5 µm; (A′ and B, right) 1 µm; (C–G) 10 µm.