PLP organizes the Cnn scaffold. (A and B) Live Cnn-mCherry in WT (A) and plp⁻ (B) embryos in interphase. Released particles (arrows), extending flare (arrowheads), and disrupted PCM (bracket) are shown. Time is given in seconds. (C and D) Embryos stained for Cnn. (E) Cnn flare length in interphase embryos; n > 80 centrosomes. Mean ± SD is indicated. (F) Cytoplasmic Cnn particles in a 100-µm² area (interphase embryos: WT n = 87, plp⁻ n = 18, PLP^Δ5 n = 24, cnn^B4 n = 21; mitotic embryos: WT n = 40, plp⁻ n = 18, PLP^Δ5 n = 9, cnn^B4 n = 15). Data are mean ± SD (error bars). ***, P < 0.001; ****, P < 0.0001 by a Student’s two-tailed t test relative to WT. Data shown are from a single representative experiment out of two or more repeats. Bars: (A and B) 2.5 µm; (C and D) 5 µm.