PLP^FL localizes to dynamic satellite structures. (A and B) Embryos were stained for the indicated proteins. Arrows show PLP at centrioles (blue) and satellites (brown). The boxed sections are enlarged below. (C) Live PLP^FL shows interphase PLP satellites (arrowheads) in NC 12 and 13. Time is given in minutes:seconds. (D) Anterograde (green) and retrograde (blue) satellite run relative to centriole (asterisk). Time is given in seconds. (D′) Corresponding kymograph and plot of distance over time (D″). (E) Average velocity of directed runs; n.s., not significant; n = 27 runs. (F) Frequency of satellites with directed (≥0.5 µm) runs; n = 58 centrosomes. Bars: (A and B, top) 5 µm; (A and B, bottom) 1 µm; (C) 5 µm; (D) 2 µm.