Reorganization of the centrosome structure in interphase. (A) SIM images of WT embryos stained for the indicated proteins. The presence (arrows) and absence (arrowheads) of γTub within Cnn flares is shown. (B) Mean radial intensity distribution of centrosome proteins in mitosis (left) and interphase (right) calculated from line scans derived from n = 30–110 centrosomes (broken lines in A). Shaded areas show the centriole (C, blue), PCM (P, orange), and flare (F, brown) zones as defined by the outer edges (OE) of Asl, γTub, and Cnn, respectively (see Materials and methods). The asterisk denotes satellite or flare measurement. (B′) Diagram of centrosome zones at mitosis (left) and interphase (right). (C) Confocal projections of the indicated proteins assayed for localization to the C, P, and F zones; +, present; −, absent; and +/−, low or variable levels; *, protein detected by GFP transgene. See Fig. S1 C for contrast-enhanced versions of Sas4, Bld10, Plk4, Polo, and Spd2. Open arrowheads show low localization of protein to the flare zone; closed arrowheads show Polo extending into the PCM zone. The brown arrowhead highlights the strong localization of PLP to the flare zone. (D) SIM image of a WT interphase centrosome with a Cnn flare (bracket); arrows show PLP at the centriole (blue) and satellites (brown). Line scan (broken line, D′) shows representative distribution relative to the centriole center. Bars: (A and D) 2.5 µm; (C) 1 µm.