Rearrangement of Cnn at the mitotic-to-interphase transition. (A) Live Cnn-GFP and H2A-RFP in a pseudo-cell (broken line) of a WT embryo through three cell cycles. Arrows show the enlarged regions below (Cnn, white). Cnn flares (red arrowheads) and separating daughter centrosomes (white arrowheads) in interphase are indicated. Bars in top panels, 5 µm. (B and C) SIM images of mitotic (B) and interphase (C) embryos stained for Cnn. Centrosomes (red boxes) are magnified to the right as projections (B′ and C′) and single optical sections (B″ and C″) through the centrosome center. Interphase flare (red arrowhead) and centriole position (white arrows) are shown. (B‴ and C‴) Cytoplasmic regions (orange boxes) show particles (open arrowheads) and a particle release event (orange arrow). (D) Live Cnn-GFP at mitotic exit. Released particles (orange) and unfolding flares (red) are shown. Time is given in minutes:seconds.