Figure 2. The centriole localization of... | Rockefeller University Press

Figure 2.

$Figure 2. The centriole localization of STIL requires Plk4. (A) Quantification of Plk4 protein levels at the centrosome of interphase cells at 24 h after addition of the indicated concentrations of IAA (gray bars). The fraction of mitotic cells that underwent successful centriole duplication (three or more centrioles) was quantified in the same samples (open bars). Bars represent the mean of >40 cells from two independent experiments. (B) Representative images of Plk4AID/AID cells immunostained with CEP192 and Centrin after treatment with the indicated dose of IAA for 24 h. Bars: (main) 5 µm; (inset) 1 µm. (C) Quantification of relative protein abundance at the centrosome of S or G2 phase (CENP-F positive) cells 1 h after IAA addition. Centriole recruitment of STIL requires Plk4. r-SAS6, rabbit SAS6 antibody; m-SAS6, mouse SAS6 antibody. Bars represent the mean of >35 cells from two independent experiments. Immunoblot shows no change in the level of endogenous STIL after Plk4 degradation. All error bars in the figure represent the SEM.$

The centriole localization of STIL requires Plk4. (A) Quantification of Plk4 protein levels at the centrosome of interphase cells at 24 h after addition of the indicated concentrations of IAA (gray bars). The fraction of mitotic cells that underwent successful centriole duplication (three or more centrioles) was quantified in the same samples (open bars). Bars represent the mean of >40 cells from two independent experiments. (B) Representative images of Plk4^AID/AID cells immunostained with CEP192 and Centrin after treatment with the indicated dose of IAA for 24 h. Bars: (main) 5 µm; (inset) 1 µm. (C) Quantification of relative protein abundance at the centrosome of S or G2 phase (CENP-F positive) cells 1 h after IAA addition. Centriole recruitment of STIL requires Plk4. r-SAS6, rabbit SAS6 antibody; m-SAS6, mouse SAS6 antibody. Bars represent the mean of >35 cells from two independent experiments. Immunoblot shows no change in the level of endogenous STIL after Plk4 degradation. All error bars in the figure represent the SEM.