Inducible destruction of endogenous Plk4. (A) Schematic outlining the strategy for the auxin-inducible destruction of Plk4. (B) Western blot showing the levels of immunoprecipitated Plk4-AID-FLAG at the indicated times after IAA addition. (C) Plk4^AID/AID cells immunostained for CEP192, Plk4, and FLAG after treatment with IAA for the indicated time. Bars: (main) 5 µm; (inset) 1 µm. (D) Quantification of the level of FLAG-tagged Plk4-AID at the centrosome at the indicated times after IAA addition. Each condition represents the mean of >70 cells from at least two independent experiments. (E) Quantification of the level of FLAG-tagged Plk4-AID at the centrosome at the indicated cell cycle stages. Horizontal line represents the mean of >70 cells from two independent experiments. (F) Quantification of Plk4 protein levels at the centrosome at the indicated time after IAA washout. Plk4 levels return to normal within 3 h of auxin removal. Each bar represents the mean of >70 cells from at least two independent experiments. All error bars in the figure represent the SEM.