Vesicular VAMP8 colocalizes with Rab11a. (A) SIM images of ectopically expressed VAMP8-TFP and mCherry-Rab5a, mCherry-Rab7a, or mCherry-Rab11a localization in unconjugated (top) and SEA-target cell-conjugated (bottom) CTLs as indicated. Bars, 2.5 µm. (B) Pearson’s coefficients for VAMP8 and markers of the various endosomal compartments (n = 11, 11, and 12 CTLs, respectively). (C) Mander’s coefficients between VAMP8 (M1) and markers of the various endosomal compartments (M2; n = 11). Error bars are means and SDs. Rest., resting; Conj., conjugated.