Depletion or overexpression of GMFβ causes changes in whole cell velocity and defects in haptotaxis. (A) Random migration velocity of single cells. Error bars represent the 10th–90th percentile. n > 60 for all conditions. Kruskal-Wallis multiple comparison testing was performed, and significance was measured with a Dunn’s post-test. ***, P < 0.001; **, P < 0.01; *, P < 0.05. (B) Rose plots (top) for CNTL (left) and GMFβ OE (right) cells migrating in a PDGF gradient. FMI (bottom left), velocity, and persistence (bottom right table) are provided. FMI is plotted as the mean ± the 95% confidence interval. Values in the table are given as the mean with 95% confidence interval. CNTL n = 89, OE n = 85. (C) Rose plots (top) for CNTL (left) and GMFβ KD (right) cells migrating in a PDGF gradient. FMI, velocity, and persistence were provided as previously described (Asokan et al., 2014). CNTL n = 118, KD n = 76. (D) Rose plots (top) for CNTL (left) and GMFβ OE (right) cells migrating in a surface-bound fibronectin gradient. FMI, velocity, and persistence were provided as previously described. CNTL n = 130, OE n = 208. (E) Rose plots (top) for CNTL (left) and GMFβ KD (right) cells migrating in a surface-bound fibronectin gradient. FMI, velocity, and persistence were provided as previously described. CNTL n = 144, KD n = 138. (F) Rose plots (top) for CNTL (left) and GMFβ KDR (right) cells migrating in a surface-bound fibronectin gradient. FMI, velocity, and persistence were provided as previously described. CNTL n = 41, KDR n = 59.