Local translation of MFA2 mRNA in the vicinity of the high-intensity granule in the shmoo. (A–C) Mfa2p-mCh protein colocalizes with its mRNA in the shmoo tip after αF treatment. WT yeast cells cotransformed with MFA2p-mCh-UA1 and UA1p-GFP (ySA147) were grown to early logarithmic phase and treated with αF for 2 h. The upper cell was photobleached, and fluorescence recovery was monitored by time-lapse microscopy. (A) Representative images from the time-lapse video before photobleaching; bar, 5 µm. The arrows point at high-intensity MFA2 mRNA granules (green)/protein (red) accumulated in the shmoo tip. (B) Immediately after photobleaching. (C) 10 min after recovery.