MFA2 mRNA granules colocalize with PBs in the shmoo tip. (A) Cells expressing MFA2 mRNA and the PB marker, Dcp2p-RFP (red; ySA22), were treated with αF for 2 h and analyzed by fluorescence microscopy. Both high- and low-intensity granules colocalized with the PB marker (see also Video 6). Arrows show high-intensity mRNA granules colocalized with PB marker; white arrowheads mark colocalized low-intensity granules; yellow arrowheads show low-intensity mRNA granules that did not colocalize with PB markers. (B) Colocalization of PGK1 mRNA and PB markers in αF-treated yeast. Cells expressing PGK1-U1A mRNA (green) and Dcp2p-RFP (red; ySA26) were treated with αF for 2 h, as indicated, and analyzed by fluorescence microscopy. (C) Colocalization of high- and low-intensity MFA2 mRNA granules with the indicated markers in αF-treated cells (ySA21; ySA23). (D) Colocalization of PGK1 mRNA with PB markers (ySA26; ySA33) in αF-treated cells. Error bars represent the standard deviation from three independent experiments. n = 220–250 cells. Bars, 5 µm.