Blocking fusion reduces the recruitment of dynein motors to AVs. (A–C) Representative images (A and B) and quantitative analysis (C) showing that blocking fusion reduces DIC recruitment to AVs. Arrows indicate AVs containing DIC-2C (A), whereas arrowheads indicate AVs not labeled with DIC-2C (B). (D and E) AVs (green) comigrate with DIC-2C in retrograde direction in control neurons (D), whereas depleting Stx17 immobilizes AVs by reducing DIC recruitment but has no effect on DIC retrograde motility along the same axons (E). The retrograde direction is toward the left. (F and G) Blocking fusion has no observable effect on DIC recruitment to LEs by quantitative analysis (C). Arrows indicate LEs containing DIC-2C. The total neuron numbers for quantification were indicated inside the bars. Error bars: SEM. Mann–Whitney test. Bars, 10 µm.