Rab27a controls Pr55^Gag recruitment to cell membranes in CD4⁺ T cells and macrophages. (A) 3D deconvolution fluorescence microscopy of HIV-1–infected PBMCs from a healthy control or a GS patient stained at day 4 p.i. with anti-p24 (green) and anti-CD81 antibodies. Shown is a 3D maximum intensity projection of 10 optical sections acquired at 0.2-µm intervals. (B) Quantitation of PM versus cytosolic distribution of Gag was evaluated by blinded observers on a per-cell basis, in 100 cells of each condition. Data are expressed as percentages of cells in each category. (C) LSCM of HIV-1–infected control or Rab27a-silenced MDMs stained at day 5 p.i. with anti-p24 (green) and anti-CD81 antibodies. (D) Quantification of Gag distribution was performed as described in B. ***, P < 0.001. KD, knockdown. Bars, 2 µm.