Inhibition of Arp2/3 complex retards bleb retraction and MLC2 recruitment. (A) Fitting curves on a per-cell basis shows that the mean maximum bleb retraction velocity decreases with increasing CK-869 dose (P < 0.001 for post-ANOVA test for linear trend). (B) MLC2 recruitment is slowed in the presence of CK-869, with the rate of recovery approximately dependent on dose (P < 0.05). Colors correspond to the concentrations shown in A. Error bars represent standard error of the mean. AU, arbitrary unit.