Figure 3.
Figure 3. Application of ADAPT to tracking cell migration. (A) Still images of HT1080 cells stably expressing GFP, taken from Video 3. Image dimensions are 1,705 × 1,368 µm, with labels representing elapsed time in hours and minutes. Bar, 150 µM. (B) Trajectories of the cells shown in Video 3, relative to the starting position of each cell (ncell = 20). (C) The influence of fibronectin concentration on the velocity and directionality of HT1080 cells. Plots show the combined data from two independent experiments. A one-way ANOVA test showed fibronectin concentration (Conc.) to have a significant impact on both measures (P < 0.0001). Error bars represent standard error of the mean.

Application of ADAPT to tracking cell migration. (A) Still images of HT1080 cells stably expressing GFP, taken from Video 3. Image dimensions are 1,705 × 1,368 µm, with labels representing elapsed time in hours and minutes. Bar, 150 µM. (B) Trajectories of the cells shown in Video 3, relative to the starting position of each cell (ncell = 20). (C) The influence of fibronectin concentration on the velocity and directionality of HT1080 cells. Plots show the combined data from two independent experiments. A one-way ANOVA test showed fibronectin concentration (Conc.) to have a significant impact on both measures (P < 0.0001). Error bars represent standard error of the mean.

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