Sac2 deletion delays cell migration. (A) Wound healing assay of ΔSac2 cells. Dashed lines measure the distance of the wound. FITC images are shown to indicate transfected cells. Bars, 20 µm. (B) Quantification of the distance traveled after 48 h as measured by the dashed lines. Data are from three replicate experiments (mean ± SEM; **, P < 0.01).