Figure 4.
Figure 4. Expression of Sac2CS mutant perturbs TfnR distribution and Tfn recycling. (A) Western blot analysis of biotin surface-labeled TfnR. (B) Densitometry analysis of the Western blot images shown in A. Data are from three replicate experiments (mean ± SEM). (C) Flow cytometry analysis of Tfn recycling in N2A cells expressing GFP (control) or GFP-Sac2CS. Data are from three replicate experiments (n = 5,000 in each time point; mean ± SEM). *, P < 0.05; **, P < 0.01.

Expression of Sac2CS mutant perturbs TfnR distribution and Tfn recycling. (A) Western blot analysis of biotin surface-labeled TfnR. (B) Densitometry analysis of the Western blot images shown in A. Data are from three replicate experiments (mean ± SEM). (C) Flow cytometry analysis of Tfn recycling in N2A cells expressing GFP (control) or GFP-Sac2CS. Data are from three replicate experiments (n = 5,000 in each time point; mean ± SEM). *, P < 0.05; **, P < 0.01.

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