Figure 2.
Figure 2. Sac2CS mutant localizes to punctate structures. (A) Schematic diagram of various constructs used in B. The C458S catalytically inactive mutation is denoted with an asterisk. (B) Confocal images of N2A cells that were transfected with GFP-Sac2, GFP-Sac2CS, or various Sac2 truncation plasmids containing an N-terminal GFP tag. (C) N2A cells were first transfected with GFP-Sac2CS for 24 h and then subjected to phenylarsine oxide (PAO; 20 µM) treatment for 10 min at 37°C. Bars, 5 µm.

Sac2CS mutant localizes to punctate structures. (A) Schematic diagram of various constructs used in B. The C458S catalytically inactive mutation is denoted with an asterisk. (B) Confocal images of N2A cells that were transfected with GFP-Sac2, GFP-Sac2CS, or various Sac2 truncation plasmids containing an N-terminal GFP tag. (C) N2A cells were first transfected with GFP-Sac2CS for 24 h and then subjected to phenylarsine oxide (PAO; 20 µM) treatment for 10 min at 37°C. Bars, 5 µm.

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