TAGLN2 controls T cell adhesion through integrin activation. (A) Representative flow cytometric profiles of conjugate formation between J-GFP or J-TG2_GFP cells and Raji B cells at indicated time points. The percentages of conjugates were calculated as described in Materials and methods. (B) J-GFP and J-TG2_GFP cells were pretreated for 30 min with or without 2 µM Cyt D, 10 µg/ml TS1/18, and 10 µg/ml TS2/4, and then, the cells were incubated for 30 min with SEE-Raji B cells. *, P < 0.05 versus J-GFP in each experimental condition. **, P < 0.05 versus J-TG2_GFP cells with SEE-loaded Raji B cells. (C) Surface expression of CD3-ε, CD28, LFA-1, and ICAM-1. Mean fluorescence intensity (MFI) of whole cell population is shown in each graph. Empty line, isotype control. (D) J-GFP and J-TG2_GFP were preincubated for 30 min with or without 10 µg/ml TS1/18 and were then applied to human ICAM-1-Fc coverslips for 30 min with or without anti-CD3/28 stimulation. Adherent cells were detected using a fluorescence plate reader. C, coated; T, treated. *, P < 0.05 versus anti-CD3/28–stimulated J-GFP cells. (E) Cells from Fig. 2 E were mixed with SEE-Raji B cells, and the percentages of conjugates were determined as in A. Flow cytometric plots shown are representative of three independent experiments, and results are the mean ± SD of triplicate experiments. *, P < 0.05 versus scrambled siRNA (si_SC) in the presence of SEE.