Far C-terminal residues of hSpindly are essential for KT localization. (A) Schematic representation of hSpindly C-terminal deletion mutants (589–605 aa). KT localization of each EGFP-tagged mutant was analyzed through fluorescence microscopy (+, KT localization; −, no KT localization). (B) Schematic representation of hSpindly C-terminal substitution mutants (597–605 aa). KT localization of each EGFP-tagged mutant was analyzed through fluorescence microscopy. (C) Representative images of HeLa cells transfected with EGFP-tagged hSpindly deletion or substitution constructs show that the C terminus of hSpindly is essential for its KT localization. Bar, 10 µm