Figure 6.
Figure 6. G3BP1 regulates SG formation in vivo. (A) MNNG shControl or shG3BP1 cells were implanted under the renal capsules of NOD/SCID mice (eight mice per group), and primary site tumors were collected 4–5 wk after implantation. Lysates extracted from tumor tissues were immunoblotted using the indicated antibodies. (B) Viable regions of shControl and shG3BP1 tumors were cryosectioned and subjected to G3BP1 IHC. Bars, 100 µm. (C) Viable regions of tumors were cryosectioned and subjected to IF with the indicated antibodies and DRAQ5 to counterstain nuclei. Insets show fivefold higher magnification of representative areas. Quantification of SGs as in Fig. 1 B is shown below as a bar graph, in which 20 high-power fields of representative tumor sections from each tumor group (n = 5) were used for SG quantification. Mean values ± SD (error bars) are shown. *, P < 0.05. Bars: (main panels) 10 µm; (enlarged insets) 1 µm.

G3BP1 regulates SG formation in vivo. (A) MNNG shControl or shG3BP1 cells were implanted under the renal capsules of NOD/SCID mice (eight mice per group), and primary site tumors were collected 4–5 wk after implantation. Lysates extracted from tumor tissues were immunoblotted using the indicated antibodies. (B) Viable regions of shControl and shG3BP1 tumors were cryosectioned and subjected to G3BP1 IHC. Bars, 100 µm. (C) Viable regions of tumors were cryosectioned and subjected to IF with the indicated antibodies and DRAQ5 to counterstain nuclei. Insets show fivefold higher magnification of representative areas. Quantification of SGs as in Fig. 1 B is shown below as a bar graph, in which 20 high-power fields of representative tumor sections from each tumor group (n = 5) were used for SG quantification. Mean values ± SD (error bars) are shown. *, P < 0.05. Bars: (main panels) 10 µm; (enlarged insets) 1 µm.

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