IFT of LIS-1, IFT velocities, and the proposed model. (A) Colocalization of GFP::LIS-1 with OSM-6::mCherry (red) in cilia (left), and a kymograph of GFP::LIS-1 motility (right). Micrograph bar, 5 µm; kymograph horizontal bar, 2 µm; vertical bar, 5 s. (B) Velocities of IFT particle and dynein subunits (mean ± SE). (C) A proposed model of subunits in the IFT–dynein complex and multiple turnaround sites on the cilium.