Transport behaviors of dynein subunits in cilia. (A) IFT frequencies of IFT particle and dynein subunits. n = 64–212; mean ± SE (error bars). ***, P ≤ 0.001. (B) Kymographs (left) of DYCI-1, DLC-1, DYLT-3, and XBX-1 in the middle (M) or distal segments (D). M″ and D″ indicate turnaround events. M and D (right) are 4× enlarged images of turnarounds in the green boxes on the left. Horizontal bar, 2 µm; vertical bar, 5 s. (C) Distribution of turnaround events along the cilia. The y axis indicates the percentage of turnaround events at the specific region of cilia among the total turnaround events. n = 58–140. The broken line shows the junction between the middle and distal segments.