Figure 2. Xfz7 signaling regulates... | Rockefeller University Press

Figure 2.

$Figure 2. Xfz7 signaling regulates Xsnail1 expression and separation behavior. (A) Quantitative analysis of tissue separation as in Fig. 1 (H and I). Mesoderm was injected as indicated (symbols as in the text). (B) In situ hybridization for Xsnail1 RNA in stage 10.5 Xenopus gastrulae; dorsal halves of sagittally fractured embryos are shown. Red arrowheads indicate blastopore; differences in morphology caused by effects of treatments on gastrulation movements. (C) RT-PCR of dorsal mesoderm from stage 10.5 gastrulae to detect Xsnail1 mRNA. (D) snail1a in situ hybridization in zebrafish. Expression is normal in uninjected and mgfp RNA–injected embryos, reduced in DN-RhoA embryo (black arrowheads), and rescued in embryo coinjected with DN-RhoA and CA-JNK mRNA. Xfz7ΔC injected into one of eight blastomeres (white arrowheads) and dvl2-MO inhibit Snail1a expression. (E) Inferred pathway of Snail1 expression control by Fz7.$

Xfz7 signaling regulates Xsnail1 expression and separation behavior. (A) Quantitative analysis of tissue separation as in Fig. 1 (H and I). Mesoderm was injected as indicated (symbols as in the text). (B) In situ hybridization for Xsnail1 RNA in stage 10.5 Xenopus gastrulae; dorsal halves of sagittally fractured embryos are shown. Red arrowheads indicate blastopore; differences in morphology caused by effects of treatments on gastrulation movements. (C) RT-PCR of dorsal mesoderm from stage 10.5 gastrulae to detect Xsnail1 mRNA. (D) snail1a in situ hybridization in zebrafish. Expression is normal in uninjected and mgfp RNA–injected embryos, reduced in DN-RhoA embryo (black arrowheads), and rescued in embryo coinjected with DN-RhoA and CA-JNK mRNA. Xfz7ΔC injected into one of eight blastomeres (white arrowheads) and dvl2-MO inhibit Snail1a expression. (E) Inferred pathway of Snail1 expression control by Fz7.