Figure 8.

Deletion of Ccq1 rescues cell death after Aurora inhibition by promoting the loading of condensin on chromosome arms. (A) Schematic representation illustrating the different localization of condensin throughout mitosis. (B) Single cell analysis of ark1-as pot1-rfp cnd1-gfp cdc11-cfp cells imaged in the presence or absence of 10 µM Napp1 (Aurora B inhibition). (C) Single cell analysis of ccq1Δ ark1-as pot1-rfp cnd1-gfp cdc11-cfp cells imaged in the presence or absence of 10 µM Napp1 (Aurora B inhibition). (D) Single cell analysis of ark1-as mis6-rfp cnd1-gfp cdc11-cfp or ark1-as pot1-rfp cnd1-gfp cdc11-cfp cells imaged in the presence of Hoechst. (E) Single cell analysis of cnd2-3E ark1-as pot1-rfp cnd1-gfp cdc11-cfp cells imaged in the presence or absence of 10 µM Napp1. In B, C, and E, the red arrows highlight the presence or absence of condensin localization to chromosome arms.

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