The role of Aurora B in telomere dispersion and disjunction requires the shelterin component Ccq1. (A) Ccq1-deleted cells were filmed through mitosis in the presence (Aurora inhibition, red, n = 92) or absence (control, black, n = 79) of 10 µM Napp1 and the number of Taz1 dots was counted according to spindle length. The data shown are from a single representative experiment out of three repeats. (B) Example of phenotypes seen in ark1-as3 ccq1Δ cells treated or not with 10 µM Napp1. (C) Percentage of anaphase chromatin bridges and merotely phenotype before or after Aurora inhibition in ccq1Δ (n = 210), cnd2-3E (n = 250), or cnd2-3A (n = 46) cells. Light gray, anaphase bridges; dark gray, merotely. Error bars indicate SD. (D) Sensitivity of wild-type, ccq1Δ, rap1Δ, and cnd2-3E mutant cells to low doses of Napp1 (0.8 µM or 1 µM) was evaluated after serial dilutions of cells on plates. Note that under these conditions ccq1Δ or cnd2-3E cells survive as opposed to wild type.