Aurora B controls the dissociation of cohesin Rad21 and Swi6/HP1 from telomeres at anaphase. (A) Rad21-gfp mis6-rfp pot1-rfp ark1-as3 cells were filmed through mitosis in the presence (Aurora inhibition, red) or absence (control, black) of 10 µM Napp1. Rad21 remains associated to telomeres as judged by its colocalization with the telomeric Pot1 signal after Aurora inhibition. (B) swi6-gfp mis6-rfp ark1-as3 cells were filmed through mitosis in the presence (Aurora inhibition, red) or absence (control, black) of 10 µM Napp1. (C) Quantification of the normalized mean fluorescence intensity of Swi6-gfp at telomeres during mitotic progression as a function of spindle length (in black) in the cells shown in A. In control mitotic cells (green), the decrease in Swi6-gfp is concomitant to the increase in spindle length during anaphase onset (black). During anaphase B, Swi6-gfp progressively reaccumulates at telomeres. In the presence of Napp1 (Aurora inhibition, red), Swi6-gfp remains constant at telomeres during spindle elongation (in black). The intensity of Swi6-gfp at telomeres in G2 cells simultaneously filmed within the same field of cells than in A is shown as a control (blue). Multiple cells were analyzed and behave in a similar manner. (D) Statistical analysis showing the mean intensity of Swi6-gfp signal at telomeres as a function of the cell cycle stage (G2, anaphase, or cytokinesis) in the presence (red) or absence (gray) of Napp1. Error bars indicate SD.