The role of Aurora B in telomere disjunction is Rap1 independent but Swi6/HP1 dependent. (A) Rap1-deleted cells were filmed through mitosis in the presence (Aurora inhibition, red, n = 139) or absence (control, black, n = 111) of 10 µM Napp1 and the number of Taz1 dots was counted according to spindle length. Note that clusters do not fully dissociate in Rap1-deleted cells as compared with wild type (light gray in the graph). The data shown are from a single representative experiment out of three repeats. (B) Example of phenotypes seen in ark1-as3 rap1Δ cells treated or not with 10 µM Napp1. (C) Swi6-deleted cells were filmed through mitosis in the presence (Aurora inhibition, red, n = 127) or absence (control, black, n = 70) of 10 µM Napp1 and the number of Taz1 dots was counted according to spindle length. The data shown are from a single representative experiment out of three repeats. (D) Example of phenotypes seen in ark1-as3 swi6Δ cells treated or not with 10 µM Napp1. (E) Percentage of anaphase chromatin bridges (light gray) and merotely phenotype (dark gray) before or after Aurora inhibition in rap1Δ or swi6Δ cells. Light gray, anaphase bridges; dark gray, merotely. Error bars indicate SD.