Telomere cluster dispersion and disjunction occurs gradually in mitosis from metaphase up to anaphase B. (A) Image of a wild-type cell expressing Taz1-gfp (telomeres, green), Cdc11-cfp (SPB, blue), and Amo1-rfp (nuclear envelope, red) during interphase. (B) Analysis of the number of Taz1 foci according to spindle length before telomere reclustering. The data shown are from a single representative experiment out of three repeats. For the experiment shown, n = 119. The red dots represent clusters of telomeres (defined as a maximum of one to three foci), whereas blue dots represent the initiation of telomere dispersion and disjunction (more than three foci). (C) Movie of a cell expressing Taz1-gfp (green) and Cdc11-cfp (blue) through mitotic progression. (D) Analysis of the cell shown in C illustrating the precise timing of telomere dispersion and disjunction according to spindle length. The number of Taz1 foci is shown with a color code for each nucleus. Multiple cells were analyzed and behave in a similar manner. Note that during phase 1 (prophase) and phase 2 (metaphase) a single nucleus is observed, whereas in phase 3 (anaphase B) the number of Taz1 foci for each nucleus is shown.